Fluorescence Imaging of Single-Copy DNA Sequences within the Human Genome Using PNA-Directed Padlock Probe Assembly
نویسندگان
چکیده
منابع مشابه
Fluorescence imaging of single-copy DNA sequences within the human genome using PNA-directed padlock probe assembly.
We present an approach for fluorescent in situ detection of short, single-copy sequences within genomic DNA in human cells. The single-copy sensitivity and single-base specificity of our method is achieved due to the combination of three components. First, a peptide nucleic acid (PNA) probe locally opens a chosen target site, which allows a padlock DNA probe to access the site and become ligate...
متن کاملVisual Detection of Bacterial Pathogens via PNA-Based Padlock Probe Assembly and Isothermal Amplification of DNAzymes
We have developed a self-reporting isothermal system for visual bacterial pathogen detection with single base resolution. The new DNA diagnostic is based on combination of peptide nucleic acid (PNA) technology, rolling circle amplification (RCA) and DNAzymes. PNAs are used as exceedingly selective chemical tools that bind genomic DNA at a predetermined sequence under nondenaturing conditions. A...
متن کاملImpedance-based detection of DNA sequences using a silicon transducer with PNA as the probe layer.
Electrochemical impedance measurements were used for the detection of single-strand DNA sequences using a peptide nucleic acid (PNA) probe layer immobilized onto Si/SiO2 chips. An epoxysilane layer is first immobilized onto the Si/SiO2 surface. The immobilization procedure consists of an epoxide/amine coupling reaction between the amino group of the PNA linker and the epoxide group of the silan...
متن کاملLocalization of trinucleotide repeat sequences in myotonic dystrophy cells using a single fluorochrome-labeled PNA probe.
A labeled peptide nucleic acid (PNA) antisense probe was used to study the spatial distribution of triplet repeats (CTG) in human myotonic dystrophy (DM) cells by high-resolution fluorescence in situ hybridization (FISH). It was found that transcripts containing triplet repeats were present as a number of discrete foci in the DM nuclei. Greater numbers of foci were visible with the PNA probe th...
متن کاملPNA Directed Genome Rare Cutting: New Developments
This chapter deals with the PNA-assisted rare cleavage (PARC) of duplex DNA. The technique is a variety of the general ‘Achilles’ heel ́ cleavage strategy and uses PNA oligomers to protect very few sites on genomic DNA against enzymatic methylation. As a result, the PARC technique makes it possible to convert common restriction enzymes into a pool of infrequent genome cutters. These artificial g...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2013
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2013.02.012